Article ID | Journal | Published Year | Pages | File Type |
---|---|---|---|---|
8085818 | Algal Research | 2018 | 7 Pages |
Abstract
In this study, rapid and direct Raman analysis of microalgae was demonstrated through measurement of algal cells (or algal paste) prepared by microwell-assisted filtration using an anodic aluminum oxide (AAO) membrane. To obtain the algal paste, an array of polydimethylsiloxane (PDMS) microwells with through holes was placed on top of the AAO membrane, after which a vacuum was initiated and a droplet of algal solution dispensed inside the PDMS microwells for a 5-min filtration process. The results showed that the AAO membrane has the lowest background noise compared to other types of filter papers or membranes. Compared to the droplet evaporation method, the “coffee-ring” effect was effectively suppressed when applying our proposed filtration method. The algal cells became stacked inside the microwells, which led to relatively uniform and strong Raman signals across individual algal paste and consistency among different algal pastes.
Related Topics
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Renewable Energy, Sustainability and the Environment
Authors
Fang-Yu Wen, Po-Sheng Chen, Ting-Wei Liao, Yi-Je Juang,