Article ID | Journal | Published Year | Pages | File Type |
---|---|---|---|---|
8086990 | Algal Research | 2016 | 9 Pages |
Abstract
The effects of temperature and nitrogen (N) on lipid productivity and fatty acid composition in three Chlorella strains grown at 20 °C, 25 °C and 30 °C in modified Tamiya media with low (7 and 21 mg Lâ 1 N) and moderate (70 mg Lâ 1 N) N were investigated. Temperature and N influenced biomass accumulation with the largest biomass accumulation (1697, 1732 and 1809 mg DW Lâ 1 for the three strains) at higher temperatures and N concentrations. Proteins decreased and lipids increased over time with N-deprivation. Strain, temperature and N concentration influenced lipid productivity with the highest productivity in cultures grown in 3% N at higher temperatures (68, 70 and 90 mg lipid Lâ 1 dayâ 1 for the three strains). The fatty acid methyl ester (FAME) profile was similar in the three strains with C16:0 > C18:1n9c > unidentified FAMEs > C18:2n6c > C18:3n3 > C18:0 comprising 90% of the total FAME. C18:1n9c and C16:0 showed the largest variation in response to culture conditions in Chlorella sp. MACC-438 (0.2-26.8% and 2.3-16.2% respectively) and C. minutissima MACC-452 (0.2-26.7% and 3.0-23.3% respectively); unidentified FAMEs (1.1-9.7%) and C18:2n6c (0.9-11.2%) were the most variable in Chlorella sp. MACC-728. Lower temperatures resulted in higher % FAME in Chlorella sp. MACC-438 (68.9%) and C. minutissima MACC-452 (91.7%). Nitrogen concentration had more influence on the specific FAME content compared to temperature. Thus, temperature tolerance is important when selecting strains to ensure high lipid productivity while the specific strains response to N-deprivation is important to ensure quality of the biofuel feedstock.
Keywords
Related Topics
Physical Sciences and Engineering
Energy
Renewable Energy, Sustainability and the Environment
Authors
Vince Ãrdög, Wendy A. Stirk, Péter Bálint, Adeyemi O. Aremu, Ambrose Okem, Csaba Lovász, Zoltán Molnár, Johannes van Staden,