Article ID | Journal | Published Year | Pages | File Type |
---|---|---|---|---|
8087953 | Algal Research | 2015 | 7 Pages |
Abstract
This study was targeted to find new sources of phycobiliproteins (PBP) as these are important as natural colours, antioxidants and fluorescent markers. Here we report Anabaena fertilissima PUPCCC 410.5 as a good producer of PBP. Control cultures of the cyanobacterium produced 383 μg PBP mgâ 1 dry biomass on day 8. The pH of the medium, supplementation of nitrogen source, sugar and illumination of cultures with different colours of light were optimized for PBP production. Optimized individual parameters were: pH of the medium 7.5; supplementation of 2 mmol nitrite Lâ 1 or 0.5% sucrose; and illumination of cultures with blue light. Total PBP production under these conditions ranged between 627 and 696 μg mgâ 1 dry biomass, resulting in nearly 1.6 fold increase, with a significant increase in phycocyanin content. Incubation of 2 mmol nitrite Lâ 1 supplemented cultures in blue light resulted in nearly 4.5 fold increase in phycoerythrin compared to the control cultures. The PBP/phycocyanin/phycoerythrin production under the optimized conditions by Anabaena fertilissima PUPCCC 410.5 is significantly higher than the PBP production by the other cyanobacterial strains reported in literature. Thus this cyanobacterial strain is a promising candidate for PBP production at commercial level.
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Authors
J.I.S. Khattar, S. Kaur, S. Kaushal, Y. Singh, D.P. Singh, S. Rana, A. Gulati,