Article ID | Journal | Published Year | Pages | File Type |
---|---|---|---|---|
8131284 | Ultrasound in Medicine & Biology | 2018 | 6 Pages |
Abstract
Chronic wounds typically require long-duration treatment with a combination of antibiotics administered systemically. This incurs adverse side effects and can require aversive surgical treatments and limb amputations. To improve non-invasive antimicrobial therapy, the objective of this study was to investigate antimicrobial chemotherapy combined with high-intensity focused ultrasound (HIFU) heating (HT). A Staphylococcus aureus abscess (80â±â30âmm3) was generated in the mouse flank region. Once the average temperature (~42â°C-46â°C) in the abscess was reached with HIFU-HT, a broad-spectrum antimicrobial (ciprofloxacin, 10âmg/kg) and perfusion marker (Evans blue dye, 40âmg/kg wt) were administered intravenously via the tail vein. Four hours later, mean abscess perfusion and colony-forming units (CFUs) per gram of abscess were determined. HIFU-HT increased abscess perfusion by ~2.5-fold (4â±â0.6âµg/mL Evans blue) compared with control (1.5â±â0.7âµg/mL), and improved antimicrobial efficacy to decrease percentage average survival of S. aureus by ~20% (46â±â7 CFUs/g of abscess) versus that seen with ciprofloxacin alone (61â±â4âCFU/g). Our in vivo data suggest that HIFU-HT can improve antimicrobial treatment responses against deep-seated bacteria in abscess wounds via enhanced perfusion.
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Authors
Rachel Wardlow, Kaustuv Sahoo, Danielle Dugat, Jerry Malayer, Ashish Ranjan,