| Article ID | Journal | Published Year | Pages | File Type |
|---|---|---|---|---|
| 8209629 | Applied Radiation and Isotopes | 2015 | 5 Pages |
Abstract
The anti-EGFR antibody Nimotuzumab was radioiodinated with I-131 by Chloramine T and Iodogen methods. The 131I-Nimotuzumab was purified and characterized by HPLC. Purified 131I-Nimotuzumab exhibited radiochemical purity of >95% and retained good in vitro stability upto 24Â h at room temperature by both the methods. Cell binding studies carried out in A431 cells expressing EGF receptors showed good immunoreactivity of the product upto 5 days post radioiodination. Biodistribution studies in normal Swiss mice showed fast clearance by both renal and gastrointestinal routes with minimal thyroid uptake.
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Authors
Mythili Kameswaran, Grace Samuel, Haladhar Dev Sarma, Swamirao N. Shinde, Ashutosh Dash, Meera Venkatesh,