Radioiodination of 2,3-dimethyl-4H-furo[3,2-c]coumarin and biological evaluation in solid tumor bearing mice

Compound 2,3-dimethyl-4H-furo[3,2-c]coumarin is a coumarin derivative that could be labeled with 125I. The process of labeling was started using 1 mg of the compound, 50 µg CAT oxidizing agent, 30 min as reaction time at pH with a yield about 95%. The 125I-coumarin derivative was stable for about 48 h. Radiochemical purity of the labeled compound was performed by electrophoresis and HPLC. The labeled compound was separated with purity about 95%. Tumor transplantation to produce a solid tumor in the right leg of albino mice was made by intramuscular injection of 2×106 EAC (Ehrlish acittes carcinoma cells). Biodistribution study of 125I-coumarin derivative revealed that the uptake in tumor bearing leg was over 5% at 1 h and 4 h post-injection. This uptake encourages the use of 123I-coumarin derivative in imaging of tumor sites.