Article ID | Journal | Published Year | Pages | File Type |
---|---|---|---|---|
8259719 | Biochimica et Biophysica Acta (BBA) - Molecular Basis of Disease | 2015 | 7 Pages |
Abstract
C/EBP homologous protein (CHOP), a transcription factor for the expression of apoptosis-related genes, plays an important role in endoplasmic reticulum (ER) stress-related organ diseases, including diseases of the kidney. Here, we investigated the role of CHOP in ischemia/reperfusion (I/R)-induced acute kidney injury using CHOP-knockout (CHOPâ/â) and wild type (CHOP+/+) mice. Fifteen or thirty minutes of bilateral renal ischemia (I/R) insult resulted in necrotic and apoptotic tubular epithelial cell death, together with increases in plasma creatinine (PCr) and blood urea nitrogen (BUN) concentrations. After I/R, BiP/GRP78 and CHOP expressions in the kidney gradually increased over time. CHOP expression was greater in the outer medulla than that in the cortex and localized intensely in the nucleus. I/R caused apoptosis of tubular epithelial cells in both CHOPâ/â and CHOP+/+ mice. The number of apoptotic cells after I/R was lower in CHOPâ/â mice than that in CHOP+/+ mice. Consistent with the degree of apoptosis, I/R-induced kidney morphological and functional damages were milder in CHOPâ/â than that in CHOP+/+ mice. The cleavage of procaspase-3 and the induction of Bax protein after I/R were lower in CHOPâ/â than that in CHOP+/+ mice. In contrast, the expression levels of Bcl-2, Bcl-xL, cIAP2, Mcl-1, and XIAP were higher in CHOPâ/â than that in CHOP+/+ mice. These results indicate that I/R induces ER stress, leading to the activation of CHOP-associated apoptosis signals, resulting in renal functional and histological damages.
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Authors
Mi Ra Noh, Jee In Kim, Sang Jun Han, Tae-Jin Lee, Kwon Moo Park,