Article ID | Journal | Published Year | Pages | File Type |
---|---|---|---|---|
8260229 | Biochimica et Biophysica Acta (BBA) - Molecular Basis of Disease | 2014 | 8 Pages |
Abstract
Mini-BALs, but not sera, from smokers show reduced concentrations of IL-33. The expression of IL-33 was increased also in bronchial epithelium from smokers. 20% CSE reduced IL-33 release but increased the mRNA for IL-33 by real time PCR and the intracellular expression of IL-33 in bronchial epithelial cells as confirmed by flow cytometry, immunocytochemistry and western blot analysis. The effect of CSE on IL-33 expression was also observed in primary bronchial epithelial cells. IL-33 expression was mainly concentrated within the cytoplasm of the cells. LPS, an agonist of TLR4, reduced IL-33 expression, and an inhibitor of TLR4 increased the intracellular expression of IL-33. In conclusion, the release of IL-33 is tightly controlled and, in smokers, an altered activation of TLR4 may lead to an increased intracellular expression of IL-33 with a limited IL-33 release.
Keywords
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Authors
Elisabetta Pace, Caterina Di Sano, Serafina Sciarrino, Valeria Scafidi, Maria Ferraro, Giuseppina Chiappara, Liboria Siena, Sebastiano Gangemi, Patrizio Vitulo, Antonino Giarratano, Mark Gjomarkaj,