Article ID | Journal | Published Year | Pages | File Type |
---|---|---|---|---|
8260298 | Biochimica et Biophysica Acta (BBA) - Molecular Basis of Disease | 2014 | 5 Pages |
Abstract
Piwi-like gene family members (Piwil 1-4) are considered stem cell-associated genes/proteins. These are expressed predominantly in germline cells, but are re-expressed in different tumors. Piwil 1-4 gene expression has not previously been studied and correlated with clinicopathological parameters in renal cell carcinomas (RCC). The Piwil 1-4 transcript levels were analyzed by quantitative real-time PCR in 73 clear cell RCC (ccRCC) tissues and corresponding normal tissues. The transcript levels of Piwil 1, 2 and 4 were strongly and significantly correlated with each other, in both the tumor tissues and the normal tissues (P < 0.001; Spearman's rank test). Piwil 4 gene expression was significantly higher in the ccRCC tissues than that in the corresponding normal renal tissues (P < 0.001; Wilcoxon signed-rank test). When the ccRCC patient cohort was divided according to the median Piwil 1-4 expression into low- and high-expression groups and according to age into younger (â¤Â 64 years) and older patient groups (> 64 years), the younger patients displayed significantly higher levels of Piwil 1 mRNA in comparison to the older patients (P = 0.010; Fisher's exact test). Interestingly, Piwil 1 expression was left-right polarized in the normal tissues but not in the tumor tissues (P = 0.004; Fisher's exact test). Altogether, associations were determined between the Piwi-like family member expression levels and clinicopathological parameters of ccRCC, suggesting a potential role for these genes/proteins in ccRCC diagnostics and tumorigenesis as well as in renal tissue embryology.
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Authors
Omar Al-Janabi, Sven Wach, Elke Nolte, Katrin Weigelt, Tilman T. Rau, Christine Stöhr, Wolfgang Legal, Stefan Schick, Thomas Greither, Arndt Hartmann, Bernd Wullich, Helge Taubert,