The hereditary spastic paraplegia protein strumpellin: Characterisation in neurons and of the effect of disease mutations on WASH complex assembly and function

► We studied the spastic paraplegia protein strumpellin, a component of WASH complex. ► The WASH complex assembles in neurons where it interacts with retromer on endosomes. ► WASH and retromer are in axons, the site of hereditary spastic paraplegia pathology. ► Strumpellin regulates the subcellular distribution of the β-2-adrenergic receptor. ► Disease mutants do not have a dominant effect on cellular functions of strumpellin.