Article ID | Journal | Published Year | Pages | File Type |
---|---|---|---|---|
8268174 | Free Radical Biology and Medicine | 2016 | 12 Pages |
Abstract
High fructose consumption has been associated to deleterious metabolic conditions. In the kidney, high fructose causes renal alterations that contribute to the development of chronic kidney disease. Evidence suggests that dietary flavonoids have the ability to prevent/attenuate risk factors of chronic diseases. This work investigated the capacity of (-)-epicatechin to prevent the renal damage induced by high fructose consumption in rats. Male Sprague Dawley rats received 10% (w/v) fructose in the drinking water for 8 weeks, with or without supplementation with (-)-epicatechin (20 mg/kg body weight/d) in the rat chow diet. Results showed that, in the presence of mild proteinuria, the renal cortex from fructose-fed rats exhibited fibrosis and decreases in nephrin, synaptopodin, and WT1, all indicators of podocyte function in association with: (i) increased markers of oxidative stress; (ii) modifications in the determinants of NO bioavailability, i.e., NO synthase (NOS) activity and expression; and (iii) development of a pro-inflammatory condition, manifested as NF-κB activation, and associated with high expression of TNFα, iNOS, and IL-6. Dietary supplementation with (-)-epicatechin prevented or ameliorated the adverse effects of high fructose consumption. These results suggest that (-)-epicatechin ingestion would benefit when renal alterations occur associated with inflammation or metabolic diseases.
Keywords
TBARSheterogeneous nuclear ribonucleoprotein A1WT1PodocyteshnRNP(–)-epicatechinMnSODnNOSeNOSCuZnSODiNOSNOSNF-κBGPXGSHCATTNFαIL-6SDS4-HNE4-hydroxynonenall-NAMEMAPKsNω-nitro-l-arginine methyl esterNADPH-oxidaseNOxsuperoxide anioninflammationNADPH oxidaseinterleukin-6tumor necrosis factor alphaSODsodium dodecyl sulfateinducible nitric oxide synthaseendothelial nitric oxide synthaseneuronal nitric oxide synthasemanganese superoxide dismutaseSuperoxide dismutasenuclear factor kappa-light-chain-enhancer of activated B cellsCopper, zinc superoxide dismutasethiobarbituric acid reactive substancesNitric oxidenitric oxide synthaseCatalasereduced glutathionemitogen-activated protein kinasesglutathione peroxidase
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Authors
Paula D. Prince, Cecilia RodrÃguez Lanzi, Jorge E. Toblli, Rosana Elesgaray, Patricia I. Oteiza, César G. Fraga, Monica Galleano,