| Article ID | Journal | Published Year | Pages | File Type |
|---|---|---|---|---|
| 8271445 | Free Radical Biology and Medicine | 2013 | 16 Pages |
Abstract
⺠This article describes enzyme-assisted derivatization for sterol analysis (EADSA). ⺠EADSA reveals nuclear receptor and G-protein-coupled receptor ligands in plasma. ⺠It is a high-sensitivity analysis for oxysterols and cholestenoic acids. ⺠Shotgun ESI-MS in negative-ion mode offers data complementary to EADSA. ⺠This methodology is illustrated on plasma from cerebrotendinous xanthomatosis patients.
Keywords
DHEACDCACTXMSNdehydroepiandrosteroneFWHMSIRQ-TOFHSDFXRMRMLXRPQDSPERICAPIESIfarnesoid X receptorG-protein-coupled receptorSolid-phase extractionSteroidSterolChenodeoxycholic acidBile acidOxysterolFourier transformLinear ion trapGirard PFree radicalsCerebrotendinous xanthomatosisselected ion recordingMass spectrometryreversed phaseLitLipidomicsDerivatizationmultiple reaction monitoringhydroxysteroid dehydrogenaseliver X receptorliquid chromatographyhigh-performance liquid chromatographyHPLCGas chromatographyReconstructed ion chromatogramNuclear receptorelectrospray ionizationAtmospheric pressure ionization
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Authors
William J. Griffiths, Peter J. Crick, Yuchen Wang, Michael Ogundare, Karin Tuschl, Andrew A. Morris, Brian W. Bigger, Peter T. Clayton, Yuqin Wang,