| Article ID | Journal | Published Year | Pages | File Type |
|---|---|---|---|---|
| 8271603 | Free Radical Biology and Medicine | 2013 | 13 Pages |
Abstract
⺠“Oxidative damage intolerance“ contributes to human autoimmunity. ⺠We describe protocols to study serum antibodies against oxidatively modified antigens. ⺠Step 1: chemically modify the antigen and physicochemically characterize the product. ⺠Step 2: immunoblot oxidatively modified antigen with patient sera or purified IgG/IgM. ⺠Step 3: measure serum autoantibodies against oxidatively modified antigen by ELISA.
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Authors
Paul Eggleton, Ahuva Nissim, Brent J. Ryan, Matthew Whiteman, Paul G. Winyard,