| Article ID | Journal | Published Year | Pages | File Type |
|---|---|---|---|---|
| 8271715 | Free Radical Biology and Medicine | 2013 | 9 Pages |
Abstract
⺠Ras is selectively modified by glutathione at cysteine 118. ⺠Ras structure is unaffected by glutathione modification at cysteine 118. ⺠Ras glutathiolation is nonactivating unless it proceeds through a free radical. ⺠Nucleotide exchange and hydrolysis of glutathiolated Ras are similar to those of wild-type Ras. ⺠Free radical-induced modifications at cysteine 118 activate Ras.
Keywords
SOS•NO2BAECGEFVSMCeNOSHSQCHCDDAFGTPase Activating ProteinROSHigher energy collisional dissociationOxidationDEANOdiaminofluoresceinFree radicalsbovine aortic endothelial cellendothelial nitric oxide synthaseSIMGAPHeteronuclear single quantum coherence spectroscopyMass spectrometryGuanine nucleotide exchange factorsselected ion monitoringNitrogen dioxideson of sevenlessGlutathioneReactive oxygen and nitrogen species
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Authors
G. Aaron Hobbs, Marcelo G. Bonini, Harsha P. Gunawardena, Xian Chen, Sharon L. Campbell,