Article ID | Journal | Published Year | Pages | File Type |
---|---|---|---|---|
8282420 | Journal of the Neurological Sciences | 2011 | 6 Pages |
Abstract
Although the importance of brain trauma as risk factor for the development of epilepsy is well established, the mechanisms of epileptogenesis are not well understood. In the present study, we revealed that the injection of a subthreshold dose of PTZ (30Â mg/Kg, i.p.) after 5Â weeks of injury induced by Fluid Percussion Brain Injury (FPI) decreased latency for first clonic seizures, increased the time of spent generalized tonic-clonic seizures and electrocorticographic (EEG) wave amplitude. In addition, statistical analysis revealed that N-acetylcysteine (NAC) (100Â mg/kg) supplementation during 5Â weeks after neuronal injury protected against behavioral and electrographical seizure activity elicited by subthreshold dose of PTZ. The supplementation of this antioxidant compound also protected against the Na+,K+-ATPase activity inhibition and concomitant increase in the levels of oxidative stress markers (protein carbonylation and thiobarbituric acid-reactive substances-TBARS) in site and peri-contusional cortical tissue. In summary, the current experiments clearly showed that FPI model induces early posttraumatic seizures and suggest that an alteration in the lipid/protein oxidation, membrane fluidity, and Na+,K+-ATPase activity may be correlated with neuronal excitability, a significant component of the secondary injury cascade that accompanies TBI.
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Authors
Luiz Fernando Almeida Silva, MaurÃcio Scopel Hoffmann, Leonardo Magno Rambo, Leandro Rodrigo Ribeiro, Frederico Diniz Lima, Ana Flavia Furian, Mauro Schneider Oliveira, Michele Rechia Fighera, Luiz Fernando Freire Royes,