| Article ID | Journal | Published Year | Pages | File Type |
|---|---|---|---|---|
| 8376365 | CMGH Cellular and Molecular Gastroenterology and Hepatology | 2018 | 20 Pages |
Abstract
Esophageal 3D organoids serve as a novel platform to investigate regulatory mechanisms in squamous epithelial homeostasis in the context of EoE and other diseases. Notch-mediated squamous cell differentiation is suppressed by cytokines known to be involved in EoE, suggesting that this may contribute to epithelial phenotypes associated with disease. Genetic and pharmacologic manipulations establish proof of concept for the utility of organoids for future studies and personalized medicine in EoE and other esophageal diseases.
Keywords
GSIKSFMOFRIVLTSLPMAML1EoEGERDBCHDAPIqRT-PCRGFPThree-dimensionalDOXEGF3-dimensional4′,6-diamidino-2-phenylindole, dihydrochlorideH&EImmunofluorescenceIHCImmunohistochemistryinterleukingastroesophageal reflux diseasetumor necrosis factor-αEMTdoxycyclineepidermal growth factorTNF-αThymic stromal lymphopoietinγ-secretase inhibitorHematoxylin and EosinBasal cell hyperplasiaquantitative reverse-transcription polymerase chain reactiongreen fluorescent proteinKeratinocytesEpithelial-mesenchymal transitioninvolucrineosinophilic esophagitis
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Authors
Yuta Kasagi, Prasanna M. Chandramouleeswaran, Kelly A. Whelan, Koji Tanaka, Veronique Giroux, Medha Sharma, Joshua Wang, Alain J. Benitez, Maureen DeMarshall, John W. Tobias, Kathryn E. Hamilton, Gary W. Falk, Jonathan M. Spergel, Andres J. Klein-Szanto,