Article ID | Journal | Published Year | Pages | File Type |
---|---|---|---|---|
8385224 | International Journal of Medical Microbiology | 2015 | 31 Pages |
Abstract
We further developed this assay towards a diagnostic tool for clinical use and evaluated its sensitivity and specificity when applied directly to human blood samples. An optimized protocol for mRNA isolation allows detection of specific CTX-M groups from as little as 100Â CFU/mL blood via reverse transcription, amplification, and pyrosequencing directly from human EDTA blood samples as well as from pre-incubated human blood cultures with a turnaround time for test results of <7Â h.
Related Topics
Life Sciences
Biochemistry, Genetics and Molecular Biology
Biochemistry, Genetics and Molecular Biology (General)
Authors
Claudia Stein, Oliwia Makarewicz, Yvonne Pfeifer, Christian Brandt, Mathias W. Pletz,