Article ID | Journal | Published Year | Pages | File Type |
---|---|---|---|---|
8386029 | International Journal of Medical Microbiology | 2011 | 9 Pages |
Abstract
Stenotrophomonas maltophilia is a microorganism of environmental and clinical importance as well as a frequent airway colonizer of cystic fibrosis (CF) individuals. We combined 2-DE and MALDI-TOF MS to profile the protein expression in S. maltophilia K279a, a completely sequenced clinical isolate, grown at 37 °C with respect to the strain grown at 26 °C. Among the proteins up-regulated at 37 °C, we identified GroEL, a molecular chaperone that mainly assist the folding and unfolding of proteins under both normal and stress conditions. A 2.4-kb groESL mRNA was detected independently by Northern blot analyses with a groES- and a groEL-specific probe, indicating that S. maltophilia groES and groEL form an operon. Primer extension analysis of S. maltophilia groESL done in Escherichia coli showed that 2 promoters, PÏ32 and PÏ70, were utilized under the heat-shock and normal condition, respectively, whereas S. maltophilia groEL was shown to act as a heat-shock gene at 37 °C, 42 °C, and, to a lesser extent, at 50 °C by real-time RT-PCR analyses. Finally, immunoblot analyses revealed that S. maltophilia GroEL strongly reacted with sera from CF patients chronically infected by the microorganism, but did not with sera from CF patients with sporadic infection or uninfected.
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Authors
Elena De Carolis, Brunella Posteraro, Ada Rita Florio, Bianca Colonna, Gianni Prosseda, Francesca Bugli, Silvia Renzetti Lorenzetti, Ersilia Fiscarelli, Rosanna Inzitari, Federica Iavarone, Massimo Castagnola, Giovanni Fadda, Maurizio Sanguinetti,