Article ID | Journal | Published Year | Pages | File Type |
---|---|---|---|---|
8389210 | Meta Gene | 2018 | 9 Pages |
Abstract
Oral submucous fibrosis (OSMF) is regarded as a collagen and collagenase metabolic disorder. Aberrant expression of matrix metalloproteinases (especially MMP-9) plays important role in remodeling of extracellular matrix (ECM) during development of OSMF. Single nucleotide polymorphisms (SNPs) in MMP-9 promoter and coding region have been demonstrated to be associated with several diseases. In this case-control study, 196 controls and 189 OSMF patients were genotyped at four MMP-9 polymorphic sites on â1562C>T, R279Q, P574R and R688Q loci by Polymerase Chain Reaction - Restriction Fragment Length Polymorphism (PCR-RFLP) method to determine the susceptibility to OSMF. The functional effect of SNPs to the development of OSMF was analyzed by studying the expression of MMP-9, collagen type-I and IV in oral biopsy tissues. R279Q SNP was found to be associated with OSMF [OR 1.5, CI (1.04-2.43), pâ¯=â¯0.03]. The 574R and 668Q alleles were significantly associated with early age group at 2.08 (pâ¯=â¯0.007) and 2.12 (pâ¯=â¯0.011) fold risk to OSMF respectively. MDR analysis showed that -1562C>T and R688Q SNPs were in strong synergy (IGâ¯=â¯0.95%, pâ¯=â¯0.0032) with increased risk of OSMF. Genotypic and functional study revealed definitive role of MMP-9 coding SNPs R279Q, P574R and R668Q in the pathogenesis of OSMF with strong predictive and prognostic value to determine OSMF at early stages in the areca chewers. Pathologically, over-expression of MMP-9 leads to decrease in collagen type-IV and epithelial thinning which contribute to basement membrane degradation along with continuous accumulation of collagen type-I enhanced by MMP-9 â1562C>T, R279Q, P574R and R688Q SNPs, resulting into early onset of OSMF.
Keywords
RFLPcross-validation consistencyOSMFCVCECMMMP-9FGFTBAMDRBCIP/NBTH&EGenetic associationepithelial to mesenchymal transitionLOXEMTTIMPLinkage disequilibriumFibroblast Growth Factorsconfidence intervalOral submucous fibrosisLysyl oxidaseExtracellular matrixMatrix metalloproteinase-9areca nutodds ratiohematoxylin/eosinpolymerase chain reactionPCRrestriction fragment length polymorphismSingle nucleotide polymorphismsSNPMultifactor dimensionality reduction
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Authors
Atul Katarkar, Chandraday Prodhan, Sanjit Mukherjee, Jay G. Ray, Keya Chaudhuri,