Article ID | Journal | Published Year | Pages | File Type |
---|---|---|---|---|
8394659 | Toxicon | 2018 | 11 Pages |
Abstract
Bothrops venezuelensis snake venoms, from five localities in the North-Central Venezuelan regions, showed biochemical and haemostatic differences. In this study, bioactivities of B. venezuelensis venoms from different regions (Aragua state; Waraira Repano (Capital District); Baruta, La Boyera and Lagunetica (Miranda state)) were compared using both natural and synthetic substrates. The protein contents of these venoms were Lagunetica 89%, La Boyera 79%, Baruta 71%, Waraira Repano 68% and Aragua 64%. Toxic activities effects were: Intraperitoneal LD50s: Aragua-14â¯mg/kg; Waraira Repano-6.4â¯mg/kg; Baruta: 8.3â¯mg/kg; La Boyera-4.4â¯mg/kg; Lagunetica-16.2â¯mg/kg. The MHD results: Aragua-21.4â¯Î¼g/mouse; Waraira Repano-2.5â¯Î¼g/mouse; Baruta-1.2â¯Î¼g/mouse; La Boyera-1.4â¯Î¼g/mouse and Lagunetica-12â¯Î¼g/mouse. The hide powder azure results: Aragua-1.24â¯U/mg; La Boyera-2.26â¯U/mg; Baruta-2.83â¯U/mg; Lagunetica-3.28â¯U/mg and Waraira Repano-5.77â¯U/mg. Esterase specific activity on BAEE results: Waraira Repano-666.66â¯U/mg; La Boyera-805.5â¯U/mg; Baruta-900.00â¯U/mg; Lagunetica-922.19â¯U/mg and Aragua-1960.67â¯U/mg. Casein zymography showed digestion bands in the molecular weight above 100 and at 66.2 and 21.5â¯kDa. Analysis of casein degradation by SDS-PAGE showed two different degradation patterns. Fibrinolytic activity (mm2/μg) on fibrin plates results: Aragua-6.07; Lagunetica-27.6; Waraira Repano-35.7; La Boyera-44.27 and Baruta-45.63. In the fibrinogenolytic assay, the five venoms completely degraded the α chain after 1â¯min of incubation. None of the venoms completely degraded the β and γ chains after 24â¯h incubation. The research indicated that venoms of B. venezuelensis of different geographic areas in Venezuela exhibit variances in composition and component concentrations; except the Aragua venom, all of them had high proteolytic activities.
Related Topics
Life Sciences
Biochemistry, Genetics and Molecular Biology
Biochemistry, Genetics and Molecular Biology (General)
Authors
MarÃa E. Girón, Vanessa Padrón, MarÃa I. Ramos, Elda E. Sánchez, Belsy Guerrero, Alberto GarcÃa, Néstor L. Uzcátegui, Luis F. Navarrete, Alexis RodrÃguez-Acosta,