Article ID | Journal | Published Year | Pages | File Type |
---|---|---|---|---|
8394720 | Toxicon | 2018 | 21 Pages |
Abstract
Lipid peroxidation, glutathione content, glutathione peroxidase activity and gene expression of transcription factors, glutathione peroxidase, glutathione synthetase and glutathione reductase was investigated in common carp liver. Short term (24â¯h) exposure of aflatoxin B1 at different doses (100, 200 and 400â¯Î¼g AFB1 kgâ1 feed) was used. It was found that conjugated dienes and trienes elevated after 16â¯h, while thiobarbituric acid reactive substances content increased only at the lowest dose after 16 and 24â¯h of exposure. Glutathione content showed higher levels than control after 16â¯h of exposure and glutathione peroxidase (GPx4) activity was higher in all of the AFB1 treated than the control group after 8â¯h of exposure. Gene expression of transcription factors showed dual response. Expression of keap1 gene down-regulated after 8â¯h and 16â¯h and nrf2 gene after16â¯h, but up-regulated after 24â¯h of exposure in the lowest, and highest dose groups. Expression of gpx4a and gpx4b genes down-regulated after 8â¯h and induction was found after 16 and 24â¯h of exposure, irrespective of the dose. The results indicated that low dose of AFB1 provokes oxidative stress earlier than higher doses, which activated the Keap1-Nrf2 pathway. At higher doses this pathway activated later, but preformed GPx4 effectively prevented lipid peroxidation.
Related Topics
Life Sciences
Biochemistry, Genetics and Molecular Biology
Biochemistry, Genetics and Molecular Biology (General)
Authors
Benjamin Kövesi, Csilla Pelyhe, Erika Zándoki, Miklós Mézes, Krisztián Balogh,