Article ID | Journal | Published Year | Pages | File Type |
---|---|---|---|---|
8395391 | Toxicon | 2015 | 5 Pages |
Abstract
Renal alterations caused by Bothrops venom and its compounds are studied to understand these effects and provide the best treatment. Previously, we studied the renal effect of the whole venom of Bothrops marajoensis and its phospholipase A2 (PLA2), but these effects could not to be attributed to PLA2. To continue the study, we report in this short communication the effects of l-amino acid oxidase from B. marajoensis venom (LAAOBm) on renal function parameter alterations observed in the same model of isolated perfused kidney, as well as the cytotoxic effect on renal cells. LAAOBm caused a decrease in PP, RVR, UF, GFR, %TNa+ and %TClâ, very similar to the effects of whole venom using the same model. We also demonstrated its cytotoxicity in MDCK cells with IC50 of 2.5 μg/mL and late apoptotic involvement demonstrated by flow cytometry assays. In conclusion, we suggested that LAAOBm is a nephrotoxic compound of B. marajoensis venom.
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Authors
Rodrigo Tavares Dantas, Antônio Rafael Coelho Jorge, Roberta Jeane Bezerra Jorge, Ramon Róseo Paula Pessoa Bezerra de Menezes, Danya Bandeira Lima, Alba FabÃola Costa Torres, Marcos Hikari Toyama, Helena Serra Azul Monteiro, Alice Maria Costa Martins,