Article ID | Journal | Published Year | Pages | File Type |
---|---|---|---|---|
8395706 | Toxicon | 2015 | 5 Pages |
Abstract
Chemical analyses of the hemagglutinating fraction from Scorpaena plumieri venom revealed that it contains five components (Sp-CL 1-5) with similar chromatographic elution profiles (35-38% of acetonitrile), molecular masses (16,800-17,000Â Da) and N-terminal sequences, suggesting that they are isoforms of the same protein. The amino acid sequence of Sp-CL4 was determined and shown to have homology with fish C-type lectins. These data demonstrate for the first time the presence of C-type isolectins in a scorpionfish venom.
Keywords
MALDI-TOFC-type lectin-like domainScorpaena plumieriCTLDCRDRP-HPLCTFAACNSPVAcetonitrileTrifluoroacetic acidSDS-PAGESodium dodecyl sulfate polyacrylamide gel electrophoresisIsolectinscarbohydrate recognition domainFish venomMass spectrometryC-type lectinMatrix assisted laser desorption ionization time-of-flightHemagglutinationReversed phase high performance liquid chromatography
Related Topics
Life Sciences
Biochemistry, Genetics and Molecular Biology
Biochemistry, Genetics and Molecular Biology (General)
Authors
F. Andrich, M. Richardson, G.B. Naumann, M.N. Cordeiro, A.V. Santos, D.M. Santos, J.S. Oliveira, M.E. de Lima, S.G. Figueiredo,