Article ID | Journal | Published Year | Pages | File Type |
---|---|---|---|---|
8397579 | Toxicon | 2013 | 7 Pages |
Abstract
In the process of evaluating the growth of Streptomyces coelicolor on rich media such as blood agar, we found that S. coelicolor a non-pathogenic, well-known antibiotic producer had the ability to grow and produce a prominent hemolytic zone. By comparing the growth with an agarase gene mutant of S. coelicolor, a similar prominent hemolytic zone was found to develop due to the organism's hemolytic activity. After the confirmation of hemolytic activity from S. coelicolor, the genome was searched for hemolysin-coding genes; consequently, SCO1782, SCO2534, and SCO3882 were identified, whose products were annotated as a putative, membrane, and hypothetical proteins, respectively. Functional characterization of all the recombinant proteins expressed in Escherichia coli BL21(DE3) revealed that only SCO1782 exhibited hemolytic activity. This S. coelicolor protein, designated as S-hemolysin, showed sequence similarity toward hemolysins from Brachyspira hyodysenteriae (35%) and Mycobacterium tuberculosis (62%). Recombinant hemolysin exhibited activity against sheep blood erythrocytes and cytolytic activity against human fibroblast cells. Deletion of SCO1782 resulted in complete loss of hemolysin activity in S. coelicolor.
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Authors
Thangamani Rajesh, Jong-Min Jeon, Yong-Hyun Kim, Hyun-Joong Kim, Da Hye Yi, Sung-Hee Park, Kwon-Young Choi, Yun-Gon Kim, Jaebum Kim, Seunho Jung, Hyung-Yeon Park, Yung-Hun Yang,