Article ID | Journal | Published Year | Pages | File Type |
---|---|---|---|---|
8397952 | Toxicon | 2013 | 4 Pages |
Abstract
A cytotoxin NN-32 (6.7Â kDa) from Indian cobra (Naja naja) venom inhibited human leukemic U937 cell growth as observed by Trypan blue dye exclusion method and cytotoxicity was confirmed by MTT assay. NN-32 induced apoptosis of U937 cell and cell cycle arrest of sub-G1 phase were revealed by FACS analysis. Increased Bax/Bcl-2 ratio, increased caspase 3 and 9 activities, cleaved PARP, decreased VEGF, MMP-2 and MMP-9 activities were observed after NN-32 treatment of U937 cell. Antileukemic activity of NN-32 on U937 cell may be due to activation of apoptosis, arresting cell cycle and antiangiogenesis activities.
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Authors
Tanaya Das, Shamik Bhattacharya, Archita Biswas, Shubho Das Gupta, Antony Gomes, Aparna Gomes,