| Article ID | Journal | Published Year | Pages | File Type |
|---|---|---|---|---|
| 8398449 | Toxicon | 2011 | 8 Pages |
Abstract
⺠Alternative methods for purification and detoxification of diphtheria toxin with polyethylene glycol activated (mPEG). ⺠Blue Sepharose chromatography was efficient for obtaining pure toxin with antigen purity 73% above the recommended level. ⺠The Purified Toxin modified with mPEG stimulated the formation of antibodies capable of neutralizing the Purified Toxin. ⺠The Purified Toxin inactivated with formaldehyde, maintained its immunogenic properties, edema formation and necrosis.
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Authors
Fernando Fratelli, José Abrahão-Neto, Aline Tojeira Prestia Caricati, Monamaris Marques Borges, Rosalvo Guidolin, Celso Pereira Caricati,