Article ID | Journal | Published Year | Pages | File Type |
---|---|---|---|---|
8398449 | Toxicon | 2011 | 8 Pages |
Abstract
⺠Alternative methods for purification and detoxification of diphtheria toxin with polyethylene glycol activated (mPEG). ⺠Blue Sepharose chromatography was efficient for obtaining pure toxin with antigen purity 73% above the recommended level. ⺠The Purified Toxin modified with mPEG stimulated the formation of antibodies capable of neutralizing the Purified Toxin. ⺠The Purified Toxin inactivated with formaldehyde, maintained its immunogenic properties, edema formation and necrosis.
Related Topics
Life Sciences
Biochemistry, Genetics and Molecular Biology
Biochemistry, Genetics and Molecular Biology (General)
Authors
Fernando Fratelli, José Abrahão-Neto, Aline Tojeira Prestia Caricati, Monamaris Marques Borges, Rosalvo Guidolin, Celso Pereira Caricati,