Article ID | Journal | Published Year | Pages | File Type |
---|---|---|---|---|
8399174 | Mitochondrion | 2015 | 11 Pages |
Abstract
Applying the algorithm we performed a pilot study in cultured motor neurons from transgenic G93A hSOD1 mice, a model of human familial amyotrophic lateral sclerosis. In the presence of the mutated SOD1 and upon excitotoxic treatment with kainate we demonstrate a clear fragmentation of the mitochondrial network, with an increase in the number of mitochondrial segments and a reduction in the length of mitochondria. Histogram analyses show a reduced number of tubular mitochondria and an increased number of small mitochondrial segments. The computer vision algorithm for the evaluation of organelle morphology allows an objective assessment of disease-related organelle phenotypes with greatly reduced examiner bias and will aid the evaluation of novel therapeutic strategies on a cellular level.
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Authors
Janin Lautenschläger, Christian Lautenschläger, Vedrana Tadic, Herbert SüÃe, Wolfgang Ortmann, Joachim Denzler, Andreas Stallmach, Otto W. Witte, Julian Grosskreutz,