Article ID | Journal | Published Year | Pages | File Type |
---|---|---|---|---|
8401328 | Progress in Biophysics and Molecular Biology | 2014 | 9 Pages |
Abstract
We developed and validated a new optical mapping system for quantification of electrical activation and repolarisation in murine atria. The system makes use of a novel 2nd generation complementary metal-oxide-semiconductor (CMOS) camera with deliberate oversampling to allow both assessment of electrical activation with high spatial and temporal resolution (128Â ÃÂ 2048 pixels) and reliable assessment of atrial murine repolarisation using post-processing of signals. Optical recordings were taken from isolated, superfused and electrically stimulated murine left atria. The system reliably describes activation sequences, identifies areas of functional block, and allows quantification of conduction velocities and vectors. Furthermore, the system records murine atrial action potentials with comparable duration to both monophasic and transmembrane action potentials in murine atria.
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Authors
Ting Yue Yu, Fahima Syeda, Andrew P. Holmes, Benjamin Osborne, Hamid Dehghani, Keith L. Brain, Paulus Kirchhof, Larissa Fabritz,