Article ID | Journal | Published Year | Pages | File Type |
---|---|---|---|---|
8407127 | Biosystems | 2014 | 14 Pages |
Abstract
Based on the experimental findings we developed a compartmental model and conducted several simulation experiments. The model simulates the mRNA expression of three different cytokines (RANTES, IL8 and TNFα) regulated by the transcription factor NFκB in mammary epithelial cells challenged with E. coli. The analysis of the gene network regulation identifies a lack of robustness and therefore sensitivity for the transcription factor regulation. However, analysis of the integrated signaling and gene network regulation model reveals distinctly different underlying mechanisms in the signaling pathway responsible for the variation between the three cytokine's mRNA expression levels. Our key findings reveal the importance of integrating the signaling pathway and gene expression dynamics in modeling. Modeling infers valid research questions which need to be verified experimentally and can assist in the design of future biological experiments.
Related Topics
Physical Sciences and Engineering
Mathematics
Modelling and Simulation
Authors
Nicoline Y. den Breems, Lan K. Nguyen, Don Kulasiri,