Characterization of epitopes recognized by monoclonal antibodies: experimental approaches supported by freely accessible bioinformatic tools

► The resolution of a mAb/antigen crystal complex is not always feasible. ► Alternative B-cell epitope mapping approaches using bioinformatic tools may be of help. ► Approaches combining experimental data generation and bioinformatics. ► Approaches exclusively based on computational analysis. ► Molecular dynamics: over the static approaches and closer to the dynamic interactions.