Article ID | Journal | Published Year | Pages | File Type |
---|---|---|---|---|
8411230 | Drug Discovery Today: Technologies | 2015 | 6 Pages |
Abstract
This review presents a new application of Resonant X-ray Emission Spectroscopy (RXES) to study the mechanism of action of metal containing anticancer derivatives and in particular platinum in situ and in vivo. The technique is an example of a photon-in photon-out X-ray spectroscopic approach, which enables chemical speciation of drugs to be determined and therefore to derive action mechanisms, and to determine drug binding rates under physiological conditions and therapeutic concentrations. This is made feasible due to the atomic specificity and high penetration depth of RXES. The review presents examples of the three main types of information that can be obtained by RXES and establishes an experimental protocol to perfect the measurements within cells.
Related Topics
Life Sciences
Biochemistry, Genetics and Molecular Biology
Biotechnology
Authors
Jacinto Sá, Joanna Czapla-Masztafiak, Ewelina Lipiec, Yves Kayser, Wojciech Kwiatek, Bayden Wood, Glen B. Deacon, Gilles Berger, François Dufrasne, Daniel L.A. Fernandes, Jakub Szlachetko,