Article ID | Journal | Published Year | Pages | File Type |
---|---|---|---|---|
8416256 | Journal of Genetic Engineering and Biotechnology | 2018 | 9 Pages |
Abstract
Proteolytic bacteria isolated from municipal solid wastes (MSW) were identified as Serratia marcescens A3 and Pseudomonas putida A2 based on 16S rDNA sequencing. Protease produced through fermentation of organic MSW by these bacteria under some optimized physicochemical parameters was partially purified and characterized. The estimated molecular mass of the partially purified protease from S. marcescens and P. putida was approximately 25 and 38 kDa, respectively. Protease from both sources showed low Km 0.3 and 0.5 mg mlâ1 and high Vmax 333 and 500 µmole minâ1 at 40â¯Â°C, and thermodynamics analysis suggested formation of ordered enzyme-substrate (E-S) complexes. The activation energy (Ea) and temperature quotient (Q10) of protease from S. marcescens and P. putida were 16.2 and 19.9 kJ/mol, and 1.4 and 1.3 at temperature range from 20 to 40 °C, respectively. Protease of the both bacterial isolates was serine and cysteine type. The protease retained approximately 97% of activity in the presence of sodium dodecyl sulphate. It was observed that the purified protease of S. marcescens could remove blood stains from white cotton cloth and degrade chicken flesh remarkably. Our study revealed that organic MSW can be used as raw materials for bacterial protease production and the protease produced by S. marcescens A3 might be potential for applications.
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Authors
Asif Iqbal, Al Hakim, Md. Saddam Hossain, Mohammad Rejaur Rahman, Kamrul Islam, Md. Faisal Azim, Jahed Ahmed, Md. Assaduzzaman, Md. Mozammel Hoq, Abul Kalam Azad,