Article ID | Journal | Published Year | Pages | File Type |
---|---|---|---|---|
8416259 | Journal of Genetic Engineering and Biotechnology | 2018 | 4 Pages |
Abstract
Altered miRNAs were associated with cigarette smoking. The study aimed to examine the gene expression level of plasma let-7a among healthy smokers and compared it with the non-smokers. Forty subjects were recruited for the present study and classified into 21 smokers and 19 non-smokers, age, and sex were matched. The software that used to design functional primers was MIRprimer. Quantitative real-time PCR was employed to compare the relative expression of plasma let-7a. Results showed that the level of let-7a was down-regulated in smokers to 0.34fold (pâ¯=â¯0.006) that of the non-smokers. Plasma let-7a showed an area under curve (AUC) of 0.749 with sensitivity 43% and specificity 100%. In conclusion, plasma let-7a was significantly down-regulated in the smokers, and it might be considered a candidate biomarker to discriminate between smokers and non-smokers.
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Authors
Sanaa A. Rizk, Fateheya M. Metwally, Asmaa M. Elfiky, Asmaa A. Mahmoud, Nadia A. Badawi, Nevin E. Sharaf, Mahmoud M. Elhefnawi,