Article ID | Journal | Published Year | Pages | File Type |
---|---|---|---|---|
8416508 | Journal of Genetic Engineering and Biotechnology | 2017 | 8 Pages |
Abstract
Abundant, low prices and a highly reduced nature make glycerol to be an ideal feedstock for the production of reduced biochemicals and biofuels. Escherichia coli has been paid much attention as the platform of microbial cell factories due to its high growth rate (giving higher metabolite production rate) and the capability of utilizing a wide range of carbon sources. However, one of the drawbacks of using E. coli as a platform is its mixed metabolite formation under anaerobic conditions. In the present study, it was shown that ethanol could be exclusively produced from glycerol by the wild type E. coli, while d-lactic acid could be exclusively produced from glucose by pflA.cra mutant, where the glucose uptake rate could be increased by this mutant as compared to the wild type strain. It was also shown that the growth rate is significantly reduced in pflA.cra mutant for the case of using glycerol as a carbon source due to redox imbalance. The metabolic regulation mechanisms behind the fermentation characteristic were clarified to some extent.
Keywords
OAAGDHGAPDHTCAAcCoADCWPEPNa2HPO4PTSMgSO4KH2PO4NaClphosphoenolpyruvic acidNaOHPFLKOHPYRcAMPNAD+Adenosine TriphosphateATPCyclic adenosine monophosphateacetyl-coenzyme Aoxaloacetic acidEscherichia coliUltra violetEthanol productionDHADihydroxyacetoneSodium chlorideBiofuelreduced form of nicotinamide adenine dinucleotideSodium phosphatephosphoenol pyruvatelactate dehydrogenaseLDHLuria Bertanimagnesium sulfateNADHnicotinamide adenine dinucleotidesodium hydroxidedry cell weightPotassium dihydrogen phosphatePotassium HydroxidecAMP receptor proteinPyruvateoptical densityglutamate dehydrogenaseGlucoseGlycerol
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Authors
Lolo Wal Marzan, Rinty Barua, Yasmin Akter, Md. Arifuzzaman, Md. Rafiqul Islam, Kazuyuki Shimizu,