Article ID | Journal | Published Year | Pages | File Type |
---|---|---|---|---|
8416580 | Journal of Genetic Engineering and Biotechnology | 2016 | 8 Pages |
Abstract
This study was carried out to investigate the effect of mannitol, sorbitol and sucrose as osmotic agents on in vitro conservation of embryogenic cultures of date palm (Phoenix dactylifera, L.) Bartamoda and Sakkoty cultivars. Embryogenic cultures was obtained using MS medium supplemented with 10Â mg/l 2,4-dichlorophenoxy acetic acid (2,4-D) and 3Â mg/l isopentenyl adenine (2iP). Among the three types of osmotic substances used for slow growth conservation, sucrose at all concentrations gave the highest percentage of survival with Sakkoty cultivar. However, addition of 40Â g/l or 60Â g/l mannitol and 20Â g/l sorbitol showed the highest percentage of survival percentage with Bartamoda cultivar. The different sucrose concentrations caused higher numbers of germinated embryos of the two cultivars compared with mannitol or sorbitol. Also, the number of germinated embryos was increased with increasing the storage periods till the ninth month. Genetic stability was determined using random amplified polymorphic DNA (RAPD) analysis. There were no clear genetic differences between the two osmotic agents used for preservation. The preserved cultures of Sakkoty cultivar gave the high percent of similarity while Bartamoda cultivar gave low percent of similarity. From the obtained results we can recommend using 40Â g/l mannitol or 20Â g/l sorbitol for in vitro preservation of Bartamoda cultivar of date palm and 20Â g/l of sucrose for Sakkoty cultivar.
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Authors
M.K. El-Bahr, A. Abd EL-Hamid, M.A. Matter, A. Shaltout, S.A. Bekheet, A.A. El-Ashry,