| Article ID | Journal | Published Year | Pages | File Type |
|---|---|---|---|---|
| 8416757 | Journal of Immunological Methods | 2018 | 5 Pages |
Abstract
Storage of intestinal tissue biopsies at â20â¯Â°C in DMSO/citrate buffer for up to 48â¯h resulted in sufficient viable cell yield for FC analysis without affecting subsequent marker-positive cell proportions. These findings support the potential shipping and storage of intestinal biopsies for centralized FC analysis in multicenter clinical trials.
Keywords
PBSSASRPMIFACSIBDPBMCBSADMSObovine serum albuminstandard deviationInflammatory bowel diseaseTwo-way analysis of varianceANOVAfluorescence-activated cell sortingRoom temperatureDimethyl sulfoxideperipheral blood mononuclear cellsStatistical Analysis SystemPhosphate buffered salineFlow cytometryRoswell Park Memorial InstituteTranslational researchMulticenter clinical trial
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Authors
Manon E. Wildenberg, Marjolijn Duijvestein, Liset Westera, Tanja van Viegen, Christianne J. Buskens, Jarmila D.W. van der Bilt, Larry Stitt, Vipul Jairath, Brian G. Feagan, Niels Vande Casteele,