Article ID | Journal | Published Year | Pages | File Type |
---|---|---|---|---|
8416782 | Journal of Immunological Methods | 2018 | 21 Pages |
Abstract
Intracellular flow cytometry showed that NK cells, T cells, and Natural Killer T (NKT) cells were producing IFNγ in the assay, however when analyzing the distribution of lymphocytes in the IFNγ-expressing subset, the proportion of NK cells far exceeded the percentage of T-, and NKT cells (pâ¯<â¯.0001). Hence, our data indicate that the readout of the test was indicative of the NK cells´ ability to mount a response and thus the results may pave the way for the assay to become applicable in the clinical setting as an estimate of NK cell activity for both diagnostic and prognostic purposes.
Keywords
APCPE-Cy7fluorescein-isothiocyanateMoAbNKTIFNγBFAFITCMFInatural killerallophycocyaninMonoclonal antibodyStandardizationinterferon-gammainterleukinbrefeldin AFrequencycytokine stimulationEnzyme-linked immunosorbent assayELISANatural killer cellsmedian fluorescence intensityphycoerythrinFlow cytometrynatural killer T
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Authors
Line Nederby, Anders Jakobsen, Marianne Hokland, Torben Frostrup Hansen,