| Article ID | Journal | Published Year | Pages | File Type |
|---|---|---|---|---|
| 8416996 | Journal of Immunological Methods | 2017 | 8 Pages |
Abstract
This new technology enables investigators to conduct large-scale primary hybridoma screens using flow cytometry. This in turn has allowed us to screen antibodies more efficiently than before and streamline identification and characterization of lead molecules.
Keywords
VioletFBSFluorescent cell barcodingHTSFCBCFSEmAbsPBSAPC5-carboxytetramethylrhodamineDMSOallophycocyaninHigh-throughputBarcodeDimethyl sulfoxidefetal bovine serumhigh-throughput screeningHybridoma screeningPhosphate buffered salineFlow cytometryMonoclonal antibodiesmultiplexcarboxyfluorescein succinimidyl ester
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Authors
Mei Lu, Brian M. Chan, Peter W. Schow, Wesley S. Chang, Chadwick T. King,