| Article ID | Journal | Published Year | Pages | File Type |
|---|---|---|---|---|
| 8417071 | Journal of Immunological Methods | 2016 | 4 Pages |
Abstract
The B6·Cg-Tg(Cd4-cre)1Cwi line expresses CRE recombinase under the control of the promoter and regulatory elements of the Cd4 gene. Here we show that CRE recombinase expression reduces the number and frequencies of CD4 positive subsets in a dose-dependent manner and localize the integration site of the transgenic construct to position 60335693-60341285 (qD) of chromosome 3. The insert contains at least 15 complete sequential copies of the transgenic construct. Based on this information we describe a novel PCR assay for genetic typing of transgenic mice.
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Authors
Kerstin Westendorf, Pawel Durek, Samia Ayew, Mir-Farzin Mashreghi, Andreas Radbruch,