| Article ID | Journal | Published Year | Pages | File Type |
|---|---|---|---|---|
| 8417348 | Journal of Immunological Methods | 2015 | 6 Pages |
Abstract
We noticed that, using a panel of 184 Caucasoid donors, a limited number of HLA alleles were incorrectly supratyped by previously described pyrosequence-based assays. Here we describe a simple implementation of the reported methods that corrects all the discrepancies found with HLA-B and -C molecular typing and allows establishing a quick and high-throughput method for the determination of HLA-Bw4 I80, Bw4T80, Bw6 and HLA-C1 or -C2 supratype.
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Authors
Biassoni R, Malnati MS, Vanni I, Raso A, Sironi F, Broccolo F, Garbarino L, Mazzocco M, Montera M, Larghero P, Di Marco E, Ugolotti E,