Processing of blood samples influences PBMC viability and outcome of cell-mediated immune responses in antiretroviral therapy-naïve HIV-1-infected patients

Article ID	Journal	Published Year	Pages	File Type
8417619	Journal of Immunological Methods	2014	10 Pages	PDF

Abstract

Our results support the need of standardized procedures for the evaluation of CMI responses, especially in HIV-1-infected, ART-naïve patients.

Keywords

Blood processing CD40-ligand PBS Staphylococcus enterotoxin B FACS CD40L TTP PBMCs CMI SAS SEB RST GMR IFN-γ IL-2 interferon-γ Interleukin-2 Viral load analysis of variance ANOVA Reverse transcriptase tumor necrosis factor-α Antiretroviral therapy fluorescence-activated cell sorting intracellular cytokine staining Cell mediated immunity peripheral blood mononuclear cells statistical analysis systems TNF-α Phosphate buffered saline geometric mean ratio ART HIV-1

Related Topics

Life Sciences Biochemistry, Genetics and Molecular Biology Biotechnology

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Processing of blood samples influences PBMC viability and outcome of cell-mediated immune responses in antiretroviral therapy-naïve HIV-1-infected patients

Authors

Patricia Bourguignon, Frédéric Clément, Frédéric Renaud, Vivien Le Bras, Marguerite Koutsoukos, Wivine Burny, Philippe Moris, Clarisse Lorin, Alix Collard, Geert Leroux-Roels, François Roman, Michel Janssens, Linos Vandekerckhove,