| Article ID | Journal | Published Year | Pages | File Type |
|---|---|---|---|---|
| 8417777 | Journal of Immunological Methods | 2014 | 8 Pages |
Abstract
Interestingly, ch14.18/CHO-mediated complement-dependent cytotoxicity (CDC) could not be detected in P2 in contrast to P3 suggesting anti-NB activity even in the presence of HACA. We showed that neither eight freeze-thaw cycles nor storage at room temperature for up to 168Â h affected HACA stability in serum. In summary, we describe a validated ELISA method suitable for the assessment of HACA in NB patients treated with ch14.18/CHO.
Keywords
mAbCDCCDRHRPIL-2TMBLTIDMEMHAHAhuman anti-chimeric antibodyanti-Idhuman anti-murine antibody3,3′,5,5′-tetramethylbenzidineBSADulbecco's modified Eagle's mediumbovine serum albuminHacAMonoclonal antibodyacetoxymethyl esterValidationstandard deviationInterleukin-2ChoChinese Hamster OvaryELISAEnzyme-linked immunosorbent assayLOD یا Limit of detectionRoom temperaturecomplement-dependent cytotoxicityAnti-idiotypeCoefficient of Variationlimit of detectionComplementarity-determining regionsNeuroblastomaHAMAHorseradish peroxidaseoptical densityquality controls
Related Topics
Life Sciences
Biochemistry, Genetics and Molecular Biology
Biotechnology
Authors
Nikolai Siebert, Christin Eger, Diana Seidel, Madlen Jüttner, Holger N. Lode,