| Article ID | Journal | Published Year | Pages | File Type |
|---|---|---|---|---|
| 8417791 | Journal of Immunological Methods | 2014 | 7 Pages |
Abstract
This report describes an assay designed to detect anti-ZnT8 autoantibodies in the serum of patients with T1D. This was carried out by generating a ZnT8 C-terminal dimer fused to the N-terminus of the Gaussia princeps luciferase that was overexpressed in insect cells using the baculovirus expression system. Recombinant protein was semi-purified and used as the target antigen in the liquid-phase luciferase immunoprecipitation system assay (LIPS), and results were compared to data obtained using a commercial ELISA designed to detect ZnT8 autoantibodies in T1D patient sera, particularly among adults. LIPS was less effective in detecting antibodies in children probably due to the relatively high prevalence of IgM anti-ZnT8 antibodies in children with T1D.
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Authors
Julia Ustinova, Eva Zusinaite, Meeme Utt, Kaja Metsküla, Koit Reimand, Vimala Huchaiah, Andres Merits, Raivo Uibo,