| Article ID | Journal | Published Year | Pages | File Type |
|---|---|---|---|---|
| 8417958 | Journal of Immunological Methods | 2013 | 8 Pages |
Abstract
A two-step solid-phase refolding method whereby Fab H-PS and Fab L-PS were successively refolded on the surface of a phi-PS plate also resulted in Fab antibody formation on the plate. Furthermore, both the binding affinity and the specificity of the Fab antibody produced by the two-step method were highly maintained, according to the results of sandwich ELISA and competitive ELISA using Fab antibody-immobilized plate via two-step solid-phase refolding. Thus, the solid-phase refolding method demonstrated in this study should be quite useful for the preparation of a Fab antibody-immobilized PS surface with high efficiency from individually produced Fab H-PS and Fab L-PS. This method will be applicable to the preparation of a large Fab antibody library on the surface of a PS plate for use in antibody screening.
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Authors
Yoichi Kumada, Kyoto Hamasaki, Aya Nakagawa, Eiju Sasaki, Tatsunori Shirai, Masahiro Okumura, Manami Inoue, Michimasa Kishimoto,