Article ID | Journal | Published Year | Pages | File Type |
---|---|---|---|---|
8418087 | Journal of Immunological Methods | 2013 | 8 Pages |
Abstract
A unique characteristic of this assay is its equal recognition of FXII and inhibitor bound FXII. This is important, as inhibitor complexes of α-FXIIa are formed in vivo as well as during blood sampling and handling. Validation of the assay demonstrated a high sensitivity, with a limit of detection and quantification of 1.2 ng/mL and 2.6 ng/mL respectively. The coefficients of variation for the repeatability and within-laboratory standard deviations were 2.6% and 5.2% respectively. The reference interval determined from healthy volunteers (n = 240) was 10.6-43 mg/L.
Keywords
kDaAPlCFXIIC1-INHTweenANCOVAHRPFPLCLOQPBSmAbC1-inhibitoro/nMonoclonal antibodyenzyme linked immunosorbent assayStreptavidinValidationstandard deviationover nightanalysis of covarianceThrombosisELISALOD یا Limit of detectionintravenouslyRoom temperaturefast protein liquid chromatographyCoefficient of VariationCoagulation factor XIIPhosphate buffered salineFibrinolysislimit of detectionlimit of quantitationHorseradish peroxidaseoptical densityplasma kallikreinKilo Dalton
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Authors
Daniel Elenius Madsen, Johannes Jakobsen Sidelmann, Kathrine Overgaard, Claus Koch, Jørgen Brodersen Gram,