| Article ID | Journal | Published Year | Pages | File Type |
|---|---|---|---|---|
| 8418237 | Journal of Immunological Methods | 2013 | 9 Pages |
Abstract
⺠We describe a method to isolate microarray-quality RNA from cells purified by FACS. ⺠Cells were sorted after fixation, permeabilization and intracellular antibody staining ⺠Despite some degradation, recovered RNA performed remarkably well in microarray. ⺠It allows gene expression profiling of samples so far limited to single-gene study.
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Authors
Maria Iglesias-Ussel, Luigi Marchionni, Fabio Romerio,