Discovery of diverse and functional antibodies from large human repertoire antibody libraries

Article ID	Journal	Published Year	Pages	File Type
8418263	Journal of Immunological Methods	2013	12 Pages	PDF

Abstract

âº Naïve human repertoire phage display Fab and scFv libraries were constructed with greater than 250Â billion members. âº These libraries mimic the natural diversity of human antibodies. âº High affinity, functional antibodies for a variety of antigens were generated without affinity maturation.

Keywords

PBS Vλ INS PBMC RCA CDR ORF scFv INSR PPE β-gal HRP TIE Vκ insulin Ang beta-galactosidase Rolling circle amplification variable light chain variable heavy chain Peripheral blood mononuclear cell Fab Phosphate buffered saline open reading frame fragment antigen binding Variable single-chain fragment variable complementary determining region framework region Phage display Horseradish peroxidase insulin receptor

Related Topics

Life Sciences Biochemistry, Genetics and Molecular Biology Biotechnology

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Discovery of diverse and functional antibodies from large human repertoire antibody libraries

Authors

Lauren J. Schwimmer, Betty Huang, Hoa Giang, Robyn L. Cotter, David S. Chemla-Vogel, Francis V. Dy, Eric M. Tam, Fangjiu Zhang, Pamela Toy, David J. Bohmann, Susan R. Watson, John W. Beaber, Nithin Reddy, Hua-Feng Kuan, Daniel H. Bedinger,