| Article ID | Journal | Published Year | Pages | File Type |
|---|---|---|---|---|
| 8418485 | Journal of Immunological Methods | 2013 | 9 Pages |
Abstract
⺠Flow cytometry was used to detect and quantify nuclear translocation. ⺠Fluorescent fusion proteins of IL-1α, IL-33 and IL-37 were detected in nuclei. ⺠Detection of IL-33 and IL-37 required fixation of nuclei while IL-1α did not. ⺠IL-1α bound stronger to nuclear structures than IL-33 or IL-37. ⺠LPS-induced nuclear translocation of IL-37 in RAW264.7 cell line started delayed.
Keywords
NF-κBNLSEYFPPFADAPISSCDTAFeGFPFSCdichlorotriazinylaminofluoresceinLPS4′,6-diamidino-2-phenylindoleNuclear translocationinterleukinInterleukin-37Interleukin-33Interleukin-1αnuclear localization sequenceFlow cytometrylipopolysaccharideparaformaldehydeforward scattersideward scatterHMGB1 proteinHigh mobility group box 1 proteinenhanced yellow fluorescent proteinenhanced green fluorescent protein
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Authors
Ralf Ross, Jan Grimmel, Sybelle Goedicke, Anna M. Möbus, Ana-Maria Bulau, Philip Bufler, Shafaqat Ali, Michael U. Martin,