| Article ID | Journal | Published Year | Pages | File Type |
|---|---|---|---|---|
| 8418486 | Journal of Immunological Methods | 2013 | 9 Pages |
Abstract
⺠A highly sensitive MAb specific for Cu2 + was produced. ⺠DF4 secreted antibody that bound tightly to Cu2 +-EDTA but not to metal-free EDTA. ⺠ELISA could be a useful tool to detect Cu2 + in environmental and human serum.
Keywords
phosphate-buffered saline containing 0.05% Tween 20PBSTKLHIC50Ci-ELISAFAASHBSTMBICP-OES3, 3′, 5, 5′-tetramethylbenzidineFBSmAbDMEMPBSTNBSADTPABSADMSObovine serum albuminMonoclonal antibodyEDTAethylenediamine tetraacetic acidstandard deviationEnzyme-linked immunosorbent assayELISADulbecco's minimal essential mediumDiethylenetriamine pentaacetic acidDimethyl sulfoxidefetal bovine serumflame atomic absorption spectroscopyCopperAffinity constantImmunoassayCross-reactivityMonoclonal antibodieskeyhole limpet hemocyanin
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Authors
Fengquan Liu, Yang Lou, Xiuying Shi, Huiming Wang, Xiaoxia Zhu,