| Article ID | Journal | Published Year | Pages | File Type |
|---|---|---|---|---|
| 8418507 | Journal of Immunological Methods | 2013 | 6 Pages |
Abstract
⺠We present complementary methods to evaluate contact hypersensitivity (CHS) in mice. ⺠Ear swelling correlates with ear weight, vascular permeability, and MPO activity in ear extracts. ⺠Concentration of IFN-γ in ear extracts confirms ear swelling data. ⺠Proliferation assay and IFN-γ production by lymph node cells support CHS results. ⺠Flow cytometry enables detection of IFN-γ+ TCRαβ CD8 CHS-effector cells.
Keywords
FCSTh1ACDIL-17DNPDNFBIFN-γIL-1βLNCCHSTc1DNP-BSASPFNKT2,4-DinitrophenolddCMPOinterferon-gammainterleukin 1-betainterleukin 17Enzyme-linked immunosorbent assayELISAContact hypersensitivityAllergic contact dermatitisfetal calf serumlymph node cellsDermal dendritic cellsNatural killer cellsmajor histocompatibility complexMHCmyeloperoxidaseVascular permeabilityspecific pathogen freeoptical density
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Authors
Magdalena Zemelka-WiÄ
cek, Monika Majewska-Szczepanik, WiesÅaw Pyrczak, Marian Szczepanik,